Modulation of Major Histocompatibility Complex-restricted presentation of nanoencapsulated ovalbumin

Зохиогч:  Г.Төрмөнх

Хамтран зохиогч:

Үйл явц: Бичиж хэвлүүлсэн

Бүтээлийн товч тайлбар: Antigen processing and presentation play a central role in the induction of specific immune responses. Various mechanisms have been proposed to explain cross-presentation. The intracellular processing pathway of ovalbumin (OVA) encapsulated with poly (DL-lactide-co-glycolide) (PLGA) was examined in the present study. In dendritic cells (DCs), class I MHC (MHC-I)-restricted presentation of OVA-nanospheres was resistant to lactacystin, a proteosome inhibitor, and brefeldin A, which blocks anterograde transport from the endoplasmic reticulum (ER) through the Golgi apparatus. Chloroquine, which inhibits phagolysomal enzymes by increasing phagolysomal pH, also inhibited MHC-I-restricted presentation of OVA-nanospheres. In addition, DCs generated from TAP-/- mice were markedly suppressed in presenting OVA-nanospheres in association with MHC-I molecules. These results demonstrate that DCs process phagocytosed OVA–nanospheres via a vacuolar alternative MHC-I pathway for the presentation of OVA peptides to T lymphocytes.

Хэвлүүлсэн газар: Chungbuk National University Press, Korea

Хэвлүүлсэн огноо: 2007/07/09

Хуудасний тоо: 136

Түлхүүр үг: #MHC-Class-II #cell interaction #MHC-Class I #antigen presentation

Бүтээл нэмсэн:Г.Төрмөнх

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